Coding

Part:BBa_K1033111:Design

Designed by: Malin Borg, Magnus Bergman, Thorstein Olaufsson, Niclas Sandberg, Nils Anlind, Pontus Danielsson, Alexander Wirtanen, Christoffer Frisk   Group: iGEM13_Uppsala   (2013-09-01)


Zeaxanthin 7,8-cleavage dioxygenase (ZCD-His) with His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 774
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose to work with a ZCD codon optimized for Synechocystis 6803.


Source

Crocus Sativus

References